Why do we need to Decalcify?

Most of us already know that decalcification is the term used for removing mineral from bones or other calcified tissue, such as cartilage. This allows for proper press processing and sectioning of specimens to be used for histological analysis.

Why is it necessary to Decalcify calcified tissues?





Decalcification is routinely used technique in most histopathological laboratories for the microscopic examination of calcified tissues [4]. The purpose of decalcification is to remove calcium salts from mineralized tissue, resulting in preservation of organic components [5].

What are the uses of decalcification?

Decalcified sections are used for the examination of bone marrow and for the diagnosis of tumours, infections, or for other purposes.

What are the tissue that require decalcification?

Any tissue containing bone, teeth, or calcifications must be FULLY FIXED prior to decalcification. Decalcification must be performed such that the tissue can be sectioned in the gross room with a blade and/or in histology with a microtome.

What happens if your pineal gland is calcified?

Morphological changes associated with pineal calcification include changes in the production of melatonin, due to the decreased function in the pineal gland parenchyma, and results in decreased pineal volume, reduced melatonin production in humans [14], and altered sleep patterns [59].

How can I make my pineal gland healthy?





Eat foods high in chlorophyl.
Dark, leafy greens are a great source of chlorophyl. Wheatgrass, blue green algae, and chlorella are also great for detoxifying the pineal gland and especially help to rid the body of fluoride. You can also find these in green superfoods supplements.

What is a good decalcifying agent?

Six decalcifying agents namely, neutral ethylene diamine tetra acetic acid (EDTA) decalcifying solution, 5% nitric acid, Perenyi’s fluid, formalin–nitric acid, 5% trichloracetic acid, and 10% formic acid were used to decalcify 24 natural teeth (four in each solution).

What is the best decalcifying agent?

Formic acid with gentle agitation produced the best decalcification overall based on time required for decalcification, ease of sectioning, hard and soft tissue staining and tissue preservation.

What is the most common decalcifying agent?

The most common acids used for decalcification are 5-10% solutions of hydrochloric acid (HCl), nitric acid, and formic acid.

What is Decalcify tissue?



Decalcification is the process of removing calcium from tissues. Calcified tissue must be decalcified before processing or the tissue cannot be sectioned. Determining when a piece of tissue is properly decalcified takes an experienced technician which is why it is best to let the IHC Core staff decalcify your tissue.

Why are my teeth Decalcifying?

When plaque is allowed to sit on the enamel for long periods of time, the bacteria in your mouth utilize the sugar from your diet to form an acid. The acid will start to dissolve or weaken the enamel and cause white decalcification spots. Eventually, the decalcified areas will lead to a cavitation and cavity.

What is the most recommended test for the completeness of decalcification?

Despite being the slowest method, EDTA at RT is still the recommended method for decalcifying mineralized tissues; however, if rapid decalcification is needed, 5% nitric acid is the best option, yielding acceptable tissue integrity and speed.

At what age does the pineal gland calcify?



Calcification of the pineal gland is typical in young adults, and has been observed in children as young as two years of age.

What stimulates the pineal gland?

Light exposure is the most important factor related to pineal gland function and melatonin secretion.

Does the pineal gland affect memory?

Neurogenesis is impaired by reduced melatonin secretion in AD. Therefore, the pineal gland dysfunction is related with the impaired neurogenesis, leading to memory loss in AD brain.

What are the symptoms of a malfunctioning pineal gland?

Some of the common signs and symptoms of a pineal tumor may include:

  • Headaches (common)
  • Nausea and vomiting.
  • Vision changes.
  • Trouble with eye movements.
  • Tiredness.
  • Memory problems.
  • Balance or coordination problems.


What happens if the pineal gland does not function properly?

If the pineal gland is impaired, it can lead to a hormone imbalance, which can affect other systems in your body. For example, sleep patterns are often disrupted if the pineal gland is impaired. This can show up in disorders such as jet lag and insomnia.

What happens when pineal gland is activated?

Stimulation of the pineal gland via its sympathetic innervation pathway results in the production of N-acetylserotonin and melatonin. Melatonin has many therapeutic roles and is heavily implicated in the regulation of the sleep-wake cycle.

How do I make my pineal gland work?

One way to control the melatonin in your body is to use melatonin supplements. These will typically make you feel tired. They may help you realign your circadian rhythm if you’ve been traveling to a different time zone or working a night shift. Supplements may also help you fall asleep faster.

What does the third eye do?



The third eye is often associated with religious visions, clairvoyance, the ability to observe chakras and auras, precognition, and out-of-body experiences. People who are said to have the capacity to utilize their third eyes are sometimes known as seers.